Welcome to the Duke Center for In Vivo Microscopy (CIVM).

The CIVM has two missions: our Small Animal Imaging Laboratory (SAIL) and Duke Imaging Innovation Laboratory (DIIL). At the heart of both is the development of innovative imaging tools and their application to important biomedical questions. We invite you to learn more here today and to contact us with questions or requests.

Duke PRATT School of Engineering

Small Animal Imaging Laboratory (SAIL)

CIVM, in collaboration with the Duke Pratt School of Engineering, provides state of the art imaging services for preclinical and basic science studies for the entire university. Featured equipment includes the 7T Bruker MRI System and the MILabs MicroSPECT System. Learn More

Duke Radiology

Duke Imaging Innovation Laboratory (DIIL)

With DIIL, CIVM is developing the next generation of imaging tools, leveraging the engineering expertise at Duke to push the technical boundaries of multimodal imaging. Featured equipment includes the 7 T Agilent MRI System and the 9.4T MRI System System. Learn More

Duke Radiology

Request a Project Collaboration

All project requests start here with your review of CIVM resources and rates and your submission of our online Project Request Form, either for SAIL or for DIIL. You'll be asked to provide the details of your project, including imaging needs, specific aims, timing and funding. Learn more and begin the project request form by choosing either SAIL or DIIL.

Recent News

CIVM congratulates Stephanie Crater!

CIVM would like to congratulate Stephanie Crater on receiving the 2021 Helmholtz Award.

Variability and heritability of mouse brain structure: Microscopic MRI atlases and connectomes for diverse strains
Genome-wide association studies have demonstrated significant links between human brain structure and common DNA… Read More
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Recent Publications

A high-resolution interactive atlas of the human brainstem using magnetic resonance imaging

Conventional atlases of the human brainstem are limited by the inflexible, sparsely-sampled, two-dimensional nature of histology, or the low spatial resolution of conventional magnetic resonance imaging (MRI). Postmortem high-resolution MRI circumvents the challenges associated with both modalities. A single human brainstem specimen extending from the rostral diencephalon through the caudal medulla was prepared for imaging after the brain was removed from a 65-year-old male within 24 h of death. The specimen was formalin-fixed for two weeks, then rehydrated and placed in a custom-made MRI compatible tube and immersed in liquid fluorocarbon. MRI was performed in a 7-Tesla scanner with 120 unique diffusion directions. Acquisition time for anatomic and diffusion images were 14 h and 208 h, respectively. Segmentation was performed manually. Deterministic fiber tractography was done using strategically chosen regions of interest and avoidance, with manual editing using expert knowledge of human neuroanatomy. Anatomic and diffusion images were rendered with isotropic resolutions of 50 μm and 200 μm, respectively. Ninety different structures were segmented and labeled, and 11 different fiber bundles were rendered with tractography. The complete atlas is available online for interactive use [insert link to https://civmvoxport.vm.duke.edu/voxbase/login.php?return_url=%2Fvoxbase%2F]. This atlas presents multiple contrasting datasets and selected tract reconstruction with unprecedented resolution for MR imaging of the human brainstem. There are immediate applications in neuroanatomical education, with the potential to serve future applications for neuroanatomical research and enhanced neurosurgical planning through “safe” zones of entry into the human brainstem.   https://doi.org/10.1016/j.neuroimage.2021.118135

Diffusion tractography of the rat knee at microscopic resolution
Wang N, Mirando AJ, Cofer G, Qi Y, Hilton MJ, Johnson GA. Diffusion tractography of the rat knee at microscopic resolution. Magn Reson Med. 2019 Jun;81(6):3775-3786. doi: 10.1002/mrm.27652. Epub 2019 Jan 22. PMID: 30671998.  https://onlinelibrary.wiley.com/doi/abs/10.1002/mrm.27652
Cytoarchitecture of the mouse brain by high resolution diffusion magnetic resonance imaging
Wang, Nian, Leonard E. White, Yi Qi, Gary Cofer, G. Allan Johnson. "Cytoarchitecture of the mouse brain by high resolution diffusion magnetic resonance imaging." NeuroImage, Volume 216, 2020, 116876, ISSN 1053-8119   https://doi.org/10.1016/j.neuroimage.2020.116876.


Characterization complex collagen fiber architecture in knee joint using high-resolution diffusion imaging
Wang, Nian, Anthony J. Mirando, Gary Cofer, Yi Qi, Matthew J. Hilton, and G Allan Johnson. “Characterization complex collagen fiber architecture in knee joint using high-resolution diffusion imaging.” Magn Reson Med, January 21, 2020. https://doi.org/10.1002/mrm.28181.




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