The Duke Center for In Vivo Microscopy was founded in 1983. It is one of the oldest preclinical imaging laboratories in the US. We have recently reinvented our mission through an exciting new collaboration between the Department of Radiology and the School of Engineering. This new partnership brings scientists from across the entire University to a single state of the art facility to invent the next generation of imaging tools through the Duke Imaging Innovation Laboratory (DIIL) and to apply those tools to pressing questions in basic and biomedical science through the service arm of the Small Animal Imaging Laboratory (SAIL).