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CIVM research

Peter Nicholls, MD - Assistant Professor of Psychiatry

Quantification of dendritic changes in real time in the CA1 region

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Dr. Nicholls is a collaborator with the Center for In Vivo Microscopy, using our Zeiss LSM 510 multiphoton confocal microscope for his studies.

The hippocampi of mice expressing a transgenic reporter of caspase-3 activity were treated with vehicle, APV, or NMDA and scanned over 4 hours by multiphoton microscopy. Each image is the sum of 15 optical slices at 10-micron intervals at a depth of 150-300 microns, scanned at a resolution of 0.8 microns per pixel. This ex vivo technique enables the quantification of dendritic changes in real time in the CA1 region.