Supplemental Material for:
Cardiovascular Phenotyping of the Mouse Heart Using a 4D Radial Acquisition and Liposomal Gd-DTPA-BMA
Elizabeth Bucholz, Ketan Ghaghada, Yi Qi, Srinivasan Mukundan, Howard A. Rockman, G. Allan Johnson
Magnetic Resonance in Medicine 63(4):979-87, 2010
Magnetic resonance (MR) microscopy has enormous potential for
small animal cardiac imaging because it is capable of producing
high-resolution images at multiple time points to accurately
measure cardiac function. To date, MR has not been used to
measure cardiac functional in the small animal because the
methods have had limited coverage and spatial resolution,
and have required relatively long scan times. Here, we
demonstrate a 4D radial acquisition in conjunction with
a liposomal blood pool agent to explore functional
differences in three populations of mice: 6 C57BL/6J mice,
6 DBA/2J mice, and 6 DBA/2J CSQ+ mice. The mice were the
same gestational age and approximately the same weight.
Cardiovascular function was determined by measuring both
left ventricular and right ventricular end diastolic volume,
end systolic volume, stroke volume, and ejection fraction.
Statistical significance was observed in end diastolic volume,
end systolic volume, and ejection fraction for left ventricular
measurements between all three populations of mice. No
statistically significant difference was observed in stroke
volume in either the left or right ventricle for any of the
three populations of mice. This study shows that MR imaging
is capable of four-dimensional cardiovascular phenotyping of
the mouse with efficient, high throughput.
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This work was performed at the Duke Center for In Vivo Microscopy,
an NCRR Biomedical Technology Research Center (P41 RR005959),
and NCI Small Animal Imaging Resource (U24 CA092656), with other
support from NHLBI R01 HL056687 to HAR). We would like to thank
Gary Cofer for his help with coil design, Sally Gewalt, and
Dr. Jiayu Song for help with reconstruction, Dr. Laurence Hedlund
for advice with animal support and handling, Jeff Brandenburg for
his help setting up the website materials, and Sally Zimney for
editorial assistance in preparing the manuscript.