Supplemental Material for:
High-Resolution Magnetic Resonance Histology of the Embryonic and Neonatal Mouse: A 4D Atlas and Morphologic Database
Alexandra Petiet, Matthew H. Kaufman, Matthew M. Goddeeris, Jeffrey L. Brandenburg, Susan A. Elmore, G. Allan Johnson
Proceedings of the National Academy of Sciences U S A 105(34):12331-12336, August 26, 2008
Engineered mice play an ever-increasing role in defining connections between
genotype and phenotypic expression. The potential of magnetic resonance
microscopy (MRM) for morphologic phenotyping in the mouse has previously
been demonstrated; however, applications have been limited by long scan
times, availability of the technology, and a foundation of normative data.
This article describes an integrated environment for high-resolution study
of normal, transgenic, and mutant mouse models at embryonic and neonatal
stages. Three-dimensional images are shown at an isotropic resolution of
19.5 microns (voxel volumes of 8 pL), acquired in 3 h at embryonic days
10.5-19.5 (10 stages) and postnatal days 0-32 (6 stages). A web-accessible
atlas encompassing this data was developed, and for critical stages of embryonic
development (prenatal days 14.5-18.5), >200 anatomical structures have been
identified and labeled. Also, matching optical histology and analysis tools
are provided to compare multiple specimens at multiple developmental stages.
The utility of the approach is demonstrated in characterizing cardiac septal
defects in conditional mutant embryos lacking the Smoothened receptor gene.
Finally, a collaborative paradigm is presented that allows sharing of data
across the scientific community. This work makes magnetic resonance microscopy
of the mouse embryo and neonate broadly available with carefully annotated
normative data and an extensive environment for collaborations.
Files in This Data Supplement
All datasets associated with this publication are available from CIVMSpace, our
Web-based data portal.
This online database is a collection of 3-dimensional MR datasets of:
It also features:
- Normal C57BL/6 mouse embryos from embryonic day E10.5 to E19.5
- Normal C57BL/6 mouse neonates from post-natal day 0 to 32
- Mutant mouse embryos with cardiac septation defects (conditional ablation of the Smoothened receptor gene, Mef2C-AHF-Cre;Smoflox/- mutants)
- Color-labeled hearts of normal and abnormal mouse embryos
- Labeled whole bodies of C57BL/6 mouse embryos, viewable in MBAT
- H&E sections of C57BL/6 mouse embryos matching MR images
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If you have trouble accessing CIVMSpace or VoxStation, please contact us at
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From CIVMSpace, select any thumbnail to browse slices from the corresponding dataset. To view
structure labels, select "Open in VoxStation" from the pull-down menu at the
upper right corner of the CIVMSpace page, then select the "Go" button; this
will launch VoxStation, a Java Web Start application that lets you view datasets
in three planes.
Below is an example of a volume dataset (C57BL/6 normal fetus at E15.5)
that can be found in CIVMSpace.
- Petiet A., Hedlund L. W., Johnson G. A., Staining methods for magnetic resonance microscopy of the rat fetus, Journal of Magnetic Resonance Imaging, 25: 1192-1198 (2007).
- Goddeeris M. M., Schwartz R., Klingensmith J., Meyers E. N., Independent requirements for Hedgehog signaling by both the anterior heart field and neural crest cells for outflow tract development, Development, 134: 1593-1604 (2007).
- Verzi Michael P., McCulley David J., De Val Sarah, Dodou Evdokia, Black Brian L., The right ventricle, outflow tract, and ventricular septum comprise a restricted expression domain within the secondary/anterior heart field, Developmental Biology, 287: 134-145 (2005).
- Zhang X. M., Ramalho-Santos M., McMahon A. P., Smoothened mutants revealed redundant roles for Shh and Ihh signaling including regulation of L/R symmetry by the mouse node, Cell, 106: 781-792 (2001).
All MR imaging was performed at the Duke Center
for In Vivo Microscopy, under NIH/NCRR grant P41 RR005959 and NCI SAIRP
grant U24 CA092656. This work was also supported by the
Mouse Bioinformatics Research Network (MBIRN), NIH/NCRR grant U24 RR021760.